Workflow How-Tos
- Progressive Deconvolution Workflow for Byosphere and Byos
- From Complex to Clear: Disulfide and Cross-Linked Peptide Analysis in Byos™
- Digested Oligo Common RNase Digest Settings
- How-to Submit a Workflow for Purity and Impurity Analysis of Synthetic Oligonucleotides Using Byos Software
- How to add plots and MS2 information to a Peptide report
- Digested Oligonucleotides workflow
Troubleshooting
- Byosphere - Unable to login: error while reading license data - incorrect format
- Issue Reporting for Byos/Byosphere desktop client
- Byonic Issue Reporting
- Byonic error message -99: No statistically significant identifications
- This application requires a Java Runtime Environment (JRE). Please install Java, either OpenJDK version > 11.0.2 or Oracle JRE version > 1.8.0
- Troubleshooting Byonic: Missing MS2 Spectra in .byrslt
Reporting
- Configuring Out-of-Range Labels in Byos Heatmap Reports
- Calculating DAR for Reduced ADCs
- Simplify Your Reporting with the New Fold Change Capability
- Oligonucleotide and Impurity UV-MS Quantitation
- Protein 3D file (pdb) configuration
- Fragment coverage map options
Intact
- Top-Down Analysis with Modified or Unnatural Amino Acids
- How to add additional protein identifications and delta masses to an Intact project
- Importing Delta masses from a CSV file
- How to reassign misassigned peaks in Intact
- Analyzing multiple ADC's in the same project
- Native MS Deconvolution Settings
Peptide How-Tos
- In-Silico Alignment in Peptide Workflows
- All-isotope XIC Quantitation in Byos
- How to use Feature Finder in Peptides-based workflows
- Generating an in-silico list of peptide masses
- How to sequence different types of cyclic peptides
- Utilizing Inclusion Lists in Peptide Searches
Peptide Insights
- Auto-decoy Setting in HCP and MPQ Workflows
- Enhancing Structural analysis with PDB files
- Cyclic Peptide Analysis
- Displaying MS2 fragment masses
- Crosslinked Peptide Pair Sequence and Score Designations in Byos
- Quantification of Modifications in the Peptide Workflows
Chromatogram & Released Glycans
- Creating Glycan Isomer Cartoons with Ambiguous Linkages
- Released Glycan Fragmentation
- How to select a single or multiple peaks in the trace plot?
- Can the released glycan workflows be used to process data acquired in positive ionization mode?
- Glycan Cartoons in Byos
- Released Glycans Tips
Application Notes
- Structural Characterization of Monoclonal Antibodies (mAb) Using Shimadzu LCMS-9030 Q-TOF and Byos Software
- Intact Reconstruction of a Lysine Conjugated ADC from Quantitative Peptide Mapping Data, Generated Using an Arginine-specific Protease
- Reliable Identification of Intra- and Inter-Molecular Cross-Links in T Cell Receptor Complexes
- Optimization of a Label-Free Peptide Quantitation Strategy for Analysis of Therapeutic Proteins: Insights from Roche's Assessment and Byos Implementation
- Multi-Attribute Methods in Byos® Part B - New Peak Detection and CQA Quantification with Feature Based Methods
- Multi-Attribute Methods in Byos® Part A – CQA Identification
Byonic
- Top-down searching using Byonic™
- Byonic Modifications list options for MSMS search
- Discriminate Leucine and Isoleucine in Byonic
- Utilizing MS/MS Filtering
- How to run Protein Metrics Preview?
- Byonic MS/MS instrument parameters
Byos General
- Sorting of the samples in projects and reports
- How to create high quality image exports
- Creating a Custom Layout
- How Do I Export Plots for Manual Annotation and Editing?
- Creating and viewing customized workflows in Byos
- How do I know there is a software update available?
Byosphere General
- Byosphere™ First Time Running Instructions for End Users
- Byosphere Metadata: Background and Applications
- Unlocking the Potential of Byosphere Dashboards through System Templates
- Opening, Editing, and Saving Byosphere Projects
- Byos to Byosphere User Transition Guide
- Byosphere Virtual and Desktop Clients
Installation & Activation
- How to install Byonic into Thermo Fisher Scientific’s Proteome Discoverer software as a node?
- Byos System Requirements
- Activate your Byos license
- Offline activation
- Activation ID copies exceeds available copies
- Offline activation/return: Response is out of order with previous responses.
Articles in Chinese (中文文章)
- 解析糖肽搜库结果
- Janssen、Genentech和Amgen创建双特异性抗体中抗体链重组高度自动化的分析方法
- Byos报告简介
- 用于多聚糖和肽图分析的自动化数据处理
- 宿主细胞蛋白 (HCP)
- Protein Metrics电荷去卷积算法简介及应用
Software Manuals
- Byos Quick Start
- Byos User Manual
- Byosphere Automated Processor Guide
- Byosphere Byos Client Manual
- Byosphere Data Uploader Guide
- Byosphere Deep Query Manual