In Standalone Byologic:
Select New project, open the Protein input tab and browse in the FASTA file (protein sequence).
Open the In-silico options tab and check the Enable in-silico box and Use table.
Click Generate peptides by digesting proteins (at the bottom), then specify the digestion enzyme and enter any modifications.
Click Generate to perform the in-silico digest:
The list of peptides will then appear and a variety of export options will be available by right clicking:
Note: if the list is exported as a csv file for use in Byos, then it's necessary to reformat the column headers.