The “Sequence and masses” input tab contains the Building blocks sub-tab.
In this sub-tab, residues can be added or changed as required to define the protein sequence. Notably, Cysteine modification options such as Carbamidomethylation (+57 Da) or Carboxymethylation (+58) can be easily selected from the three provided choices. Upon selection, the chemical formula will be adjusted accordingly
Figure : Unmodified cysteine (default)
Figure 3: Carbamidomethylated cysteine
Figure : Carboxymethylated cysteine
NOTE: this feature affects all Cysteines (C) within the computed sequence mass.